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BBa_K1758201

BBa_K1758201 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1758201

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Team Bielefeld-CeBiTec 2015

Date created: 2015-08-27 11:00:00

Date modified: 2015-09-18 09:11:15

His-tagged lac repressor under the control of the T7 promoter

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Details

Types	DnaRegion
Roles	Generator engineered_region
Sequences	BBa_K1758201_sequence (Version 1)

Description

Uses T7 promotor and a strong rbs (BBa_K525998) and the lac repressor with a His-Tag. For a higher production of lacI we combine the gene for lacI with T7-RBS (Protein expression can be induced via rhamnose). By adding a His-Tag, lacI can be purified via Ni-NTA columns.

Notes

This is a composite part which enables a high protein expression and an easy purification of lacI.

Source

Parts from the parts registry are combined and a His-Tag is added through PCR and Gibson Assembly.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K525998 T7 promoter BBa_B0034 RBS lacI His-Tag	1,32 1,20 21,32 24,27 39,1127 1128,1151	feature/BioBrick engineered_region promoter feature/promoter feature/BioBrick engineered_region ribosome_entry_site feature/rbs CDS feature/cds tag feature/tag