Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jiayi Dou, Hao Jiang
Date created: 2009-08-12 11:00:00
Date modified: 2015-05-08 01:11:02
Reverse primer VR6 (K176050) binding site
| Types | DnaRegion |
| Roles | DNA sequence_feature |
| Sequences | BBa_K176040_sequence (Version 1) |
Description
This part can be ligated into composite parts as a primer binding site for PCR identification of the part or sequencing of the part. It will be very useful when many composite parts have similar lengths, then PCR using VF2 and VR cannot be used to identify the parts.Reverse primers VR3~VR12 are designed with our software tool, which is modified from primer3 to pick primers from random sequences for synthetic biological primer design. These primers are designed to be perfectly paired with VF2 and without cross-reactivities to each other.
Notes
Designed with our software tool, which is modified from primer3 to pick primers from random sequences for synthetic biological primer design.Main parameters for picking VR3~VR12:
Size: 20 bp (N19 + G or C)
Tm: VF2 Tm ??1℃
GC%: 50%
Max Self Complementarity: 3 bp
Max 3' Self Complementarity: 2 bp
Max Pair Complementarity: 3 bp
Max 3' Pair Complementarity: 2 bp
Max 3' Stability: 3.36 kcal/mol
Max Template(pSB1A3) Mispriming: 6 bp
Max Mispriming With Other VR3~VR12 Sites: 4 bp