VR10 bindi

BBa_K176044 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K176044
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jiayi Dou, Hao Jiang
Date created: 2009-08-12 11:00:00
Date modified: 2015-05-08 01:11:02

Reverse primer VR10 (K176054) binding site



Types
DnaRegion

Roles
DNA

sequence_feature

Sequences BBa_K176044_sequence (Version 1)

Description

This part can be ligated into composite parts as a primer binding site for PCR identification of the part or sequencing of the part. It will be very useful when many composite parts have similar lengths, then PCR using VF2 and VR cannot be used to identify the parts.

Reverse primers VR3~VR12 are designed with our software tool, which is modified from primer3 to pick primers from random sequences for synthetic biological primer design. These primers are designed to be perfectly paired with VF2 and without cross-reactivities to each other.

Notes

Designed with our software tool, which is modified from primer3 to pick primers from random sequences for synthetic biological primer design.

Source

Reverse complement of VR10 (K176054). Picked from random sequences.

Sequence Annotation Location Component / Role(s)
VR10
1,20
feature/primer_binding primer_binding_site
igem#experience
Works
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K176044/1