VR10 bindi

BBa_K176044 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K176044

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jiayi Dou, Hao Jiang

Date created: 2009-08-12 11:00:00

Date modified: 2015-05-08 01:11:02

Reverse primer VR10 (K176054) binding site

Details

• Types: DnaRegion
• Roles: DNA; sequence_feature
• Sequences: BBa_K176044_sequence (Version 1)

Description

This part can be ligated into composite parts as a primer binding site for PCR identification of the part or sequencing of the part. It will be very useful when many composite parts have similar lengths, then PCR using VF2 and VR cannot be used to identify the parts.

Reverse primers VR3~VR12 are designed with our software tool, which is modified from primer3 to pick primers from random sequences for synthetic biological primer design. These primers are designed to be perfectly paired with VF2 and without cross-reactivities to each other.

Notes

Designed with our software tool, which is modified from primer3 to pick primers from random sequences for synthetic biological primer design.

Source

Reverse complement of VR10 (K176054). Picked from random sequences.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
VR10	1,20	feature/primer_binding, primer_binding_site

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K176044/1

Member of these Collections

• iGEM Parts Registry