VR5

BBa_K176049 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K176049

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jiayi Dou, Hao Jiang

Date created: 2009-08-12 11:00:00

Date modified: 2015-05-08 01:11:02

Reverse primer VR5 (bind to K176039), member of VR3~VR12

Details

• Types: DnaRegion
• Roles: primer; Primer
• Sequences: BBa_K176049_sequence (Version 1)

Description

This part binds to K176039, which can be ligated into composite parts as a primer binding site for PCR identification of the part or sequencing of the part. It will be very useful when many composite parts have similar lengths, then PCR using VF2 and VR cannot be used to identify the parts.

Reverse primers VR3~VR12 are designed with our software tool, which is modified from primer3 to pick primers from random sequences for synthetic biological primer design. These primers are designed to be perfectly paired with VF2 and without cross-reactivities to each other.

Notes

Designed with our software tool, which is modified from primer3 to pick primers from random sequences for synthetic biological primer design.

Source

Reverse complement of K176039. Picked from random sequences.

Other Properties

• igem#experience: Works
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K176049/1

Member of these Collections

• iGEM Parts Registry