ccdB-LVA

BBa_K176157 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_K176157

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Danqian Liu, Zongxiao He, Hao Jiang

Date created: 2009-09-13 11:00:00

Date modified: 2015-07-09 11:35:55

ccdB-LVA coding sequence

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K176157_sequence (Version 1)

Description

Add LVA degradation tag (widely used in C0040, C0051, C0061...) to ccdB(K145151) C-terminal:

[K145151 without stop codon] GCT GCA AAC GAC GAA AAC TAC GCT TTA GTA GCT [TAA TAA]

A A N D E N Y A L V A

Notes

Add LVA degradation tag (widely used in C0040, C0051, C0061...) to ccdB(K145151) C-terminal:

[K145151 without stop codon] GCT GCA AAC GAC GAA AAC TAC GCT TTA GTA GCT [TAA TAA]

A A N D E N Y A L V A

Source

PCR from K145151.

Forward Primer: VF2(G00100)

Reverse Primer: GTTT ACTAGT A TTATTA AGC TAC TAA AGC GTA GTT TTC GTC GTT TGC AGC tattccccagaacatcaggtta

Digest with EcoRI and SpeI, ligate into pSB1A3.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
ccdB (K145151)	1,303	CDS, feature/cds
LVA degradation tag (AANDENYALVA)	304,336	tag, feature/tag
Start	1,3	start_codon, feature/start
Stop	337,342	stop_codon, feature/stop
VRccdB primer (K176058)	17,44	primer_binding_site, feature/primer_binding

Other Properties

• igem#experience: Issues
• igem#sampleStatus: It's complicated
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K176157/1

Member of these Collections

• iGEM Parts Registry