Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Danqian Liu, Zongxiao He, Hao Jiang
Date created: 2009-10-07 11:00:00
Date modified: 2015-05-08 01:11:04
Cell density controller: pCon 0.04->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB-LVA(weak RBS)
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K176212_sequence (Version 1) |
Description
Cell Death = (Cell Density * pCon 0.04) + Input AHLNotes
Input: AHLOutput1: AHL
Output2: Cell Density
Design:
pCon 0.04(J23109) generates LuxI-LVA, LuxI-LVA generates Output1 AHL, the concentration of AHL in the medium depends on both LuxI-LVA level per cell and cell density.
pCon 0.70(J23101) generates LuxR.
pLux/Tet(K176000) generates lacZalpha-ccdB-LVA(weak RBS) to kill the cell, activated by LuxR and (Output1 AHL generated by LuxI-LVA + Input AHL), repressed by TetR and no aTc.
Source
K176086 K176166| Access | Instance | Definition |
| public public | BBa_K176086 BBa_K176166 | BBa_K176086 BBa_K176166 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K176086 BBa_K176166 | 1,1908 1917,2586 | BBa_K176086 BBa_K176166 |