BBa_K176214

BBa_K176214 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K176214
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Danqian Liu, Zongxiao He, Hao Jiang
Date created: 2009-10-07 11:00:00
Date modified: 2015-05-08 01:11:04

Cell density controller: pCon 0.01->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB-LVA



Types
DnaRegion

Roles
engineered_region

Device

Sequences BBa_K176214_sequence (Version 1)

Description

Cell Death = (Cell Density * pCon 0.01) + Input AHL

Notes

Input: AHL

Output1: AHL

Output2: Cell Density

Design:

pCon 0.01(J23103) generates LuxI-LVA, LuxI-LVA generates Output1 AHL, the concentration of AHL in the medium depends on both LuxI-LVA level per cell and cell density.

pCon 0.70(J23101) generates LuxR.

pLux/Tet(K176000) generates lacZalpha-ccdB-LVA to kill the cell, activated by LuxR and (Output1 AHL generated by LuxI-LVA + Input AHL), repressed by TetR and no aTc.

Source

K176087 K176164

igem#experience
Fails
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
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user/james
 
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synbiohub#topLevel
BBa_K176214/1