BBa_K176216

BBa_K176216 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K176216
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Danqian Liu, Zongxiao He, Hao Jiang
Date created: 2009-10-07 11:00:00
Date modified: 2015-05-08 01:11:04

Cell density controller: pCon 0.01->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB-LVA(weak RBS)



    • Details

    Types
    DnaRegion

    Roles
    Device

    engineered_region

    Sequences BBa_K176216_sequence (Version 1)

    Description

    Cell Death = (Cell Density * pCon 0.01) + Input AHL

    Notes

    Input: AHL

    Output1: AHL

    Output2: Cell Density

    Design:

    pCon 0.01(J23103) generates LuxI-LVA, LuxI-LVA generates Output1 AHL, the concentration of AHL in the medium depends on both LuxI-LVA level per cell and cell density.

    pCon 0.70(J23101) generates LuxR.

    pLux/Tet(K176000) generates lacZalpha-ccdB-LVA(weak RBS) to kill the cell, activated by LuxR and (Output1 AHL generated by LuxI-LVA + Input AHL), repressed by TetR and no aTc.

    Source

    K176087 K176166

    Access Instance Definition
    public
    public
    		BBa_K176087
    BBa_K176166
    		BBa_K176087
    BBa_K176166

    Sequence Annotation Location Component / Role(s)
    BBa_K176087
    BBa_K176166
    		1,1908
    1917,2586
    		BBa_K176087
    BBa_K176166

    igem#experience
    Fails
     
    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K176216/1
     

    Attachments

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