BBa_K176221

BBa_K176221 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K176221

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Danqian Liu, Zongxiao He, Hao Jiang

Date created: 2009-10-07 11:00:00

Date modified: 2015-05-08 01:11:04

cell density->GFP: pCon 0.01->luxI-LVA+pCon 0.70->luxR+pLux/Tet->GFP

Details

• Types: DnaRegion
• Roles: engineered_region; Device
• Sequences: BBa_K176221_sequence (Version 1)

Description

GFP = (Cell Density * pCon 0.01) + Input AHL

High level GFP inhibits cell growth.

This part is both a cell density reporter and a cell density controller.

Notes

Input: AHL

Output1: AHL

Output2: GFP

Output3: Cell Density

Design:

pCon 0.01(J23103) generates LuxI-LVA, LuxI-LVA generates Output1 AHL, the concentration of AHL in the medium depends on both LuxI-LVA level per cell and cell density.

pCon 0.36(K176009) generates LuxR.

pLux/Tet(K176000) generates Output2 GFP, activated by LuxR and (Output1 AHL generated by LuxI-LVA + Input AHL), repressed by TetR and no aTc. High level GFP inhibits cell growth to control Output3 Cell Density.

Source

K176024 K176126

Components

Access	Instance	Definition
public	BBa_K176087	BBa_K176087
public	BBa_I13504	BBa_I13504

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K176087	1,1908	BBa_K176087
BBa_I13504	1917,2791	BBa_I13504

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/myers
• synbiohub#ownedBy: user/james
• synbiohub#topLevel: BBa_K176221/1

Member of these Collections

• iGEM Parts Registry