Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Danqian Liu, Zongxiao He, Hao Jiang
Date created: 2009-10-12 11:00:00
Date modified: 2015-05-08 01:11:04
Repressible cell density controller: PoPS->tetR-LVA->K176098
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K176227_sequence (Version 1) |
Description
PoPS->tetR-LVA+pCon 0.01->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)Cell Death = ((Cell Density * pCon 0.01) + Input AHL) / (1 + Input PoPS)
High Input PoPS -> High Steady State Cell Density
High Input AHL -> Low Steady State Cell Density
Notes
PoPS->tetR-LVA+pCon 0.01->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)Input1: PoPS
Input2: AHL
Output1: AHL
Output2: Cell Density
Design:
Input1 PoPS generates TetR-LVA.
pCon 0.01(J23103) generates LuxI-LVA, LuxI-LVA generates Output1 AHL, the concentration of AHL in the medium depends on both LuxI-LVA level per cell and cell density.
pCon 0.70(J23101) generates LuxR.
pLux/Tet(K176000) generates lacZalpha-ccdB (weak RBS) to kill the cell, activated by LuxR and (Output1 AHL generated by LuxI-LVA + Input2 AHL), repressed by TetR-LVA and no aTc.
Source
K176006 K176098| Access | Instance | Definition |
| public public | BBa_K176006 BBa_K176098 | BBa_K176006 BBa_K176098 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K176006 BBa_K176098 | 1,840 849,3401 | BBa_K176006 BBa_K176098 |