BBa_K176237

BBa_K176237 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K176237

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Chao Li, Hao Jiang

Date created: 2009-10-13 11:00:00

Date modified: 2015-05-08 01:11:04

Repressible cell density->PoPS: PoPS->tetR-LVA->K176085->PoPS

Details

• Types: DnaRegion
• Roles: engineered_region; Device
• Sequences: BBa_K176237_sequence (Version 1)

Description

PoPS->tetR-LVA + pCon 0.15->luxI-LVA + pCon 0.70->luxR + pLux/Tet->PoPS

Output PoPS = ((Cell Density * pCon 0.15) + Input AHL) / (1 + Input PoPS)

High Input PoPS -> Low Output PoPS

High Input AHL -> High Output PoPS

Notes

PoPS->tetR-LVA + pCon 0.15->luxI-LVA + pCon 0.70->luxR + pLux/Tet->PoPS

Input1: PoPS

Input2: AHL

Output1: AHL

Output2: PoPS

Design:

Input1 PoPS generates TetR-LVA.

pCon 0.15(K176008) generates LuxI-LVA, LuxI-LVA generates Output1 AHL, the concentration of AHL in the medium depends on both LuxI-LVA level per cell and cell density.

pCon 0.70(J23101) generates LuxR.

pLux/Tet(K176000) generates Output2 PoPS, activated by LuxR and (Output1 AHL generated by LuxI-LVA + Input2 AHL), repressed by TetR-LVA and no aTc.

Source

K176006 K176085

Components

Access	Instance	Definition
public	BBa_K176006	BBa_K176006
public	BBa_K176085	BBa_K176085

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K176006	1,840	BBa_K176006
BBa_K176085	849,2756	BBa_K176085

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K176237/1

Member of these Collections

• iGEM Parts Registry