Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Vinson Lam
Date created: 2015-06-01 11:00:00
Date modified: 2015-09-16 10:22:49
Major Spidroin Protein 2 (MaSp2) with sticky ends AB
| Types | DnaRegion |
| Roles | sequence_feature DNA |
| Sequences | BBa_K1763002_sequence (Version 1) |
Description
This part contains the core sequence of MaSp2 that has been assembled with a 5'-AGTT-3' overhang on the 5' end of the sequence and 3'-ACAG-5' overhang on the 3' end of the sequence. This part was designed for use with ICA, along with parts BBa_K1763003 and BBa_K1763004 as an efficient method to assemble multiple MaSp2 sequences together. This setup is to show that this particular set of sticky ends is suitable for ICA. Before using in ICA, this part should be digested with BsaI, a type IIs restriction enzyme, which cuts DNA outside of its recognition site.Notes
The gene sequence for MaSp2 is particularly GC-rich, which may complicate PCR.Source
This part is derived form the MaSp2 gene found in the spider N. Clavipes.| Sequence Annotation | Location | Component / Role(s) |
| A sticky end BsaI recognition site (reverse) BsaI recognition site (forward) B sticky end MaSp2 Core sequence | 7,10 114,119 1,5 109,112 11,108 | sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/dna |