Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: iGEM Technion HS
Date created: 2015-09-14 11:00:00
Date modified: 2015-09-17 02:05:48
P(Lac)IQ RBS Aiia RBS LuxR term term luxpR
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1767001_sequence (Version 1) |
Description
We designed and built a kill switch mechanism that is based on the activation of a lethal gene downstream of an inducible promoter that is activated over time. The presented part is the first module of our designed genetic circuit (BBa_K1767003). In order to characterize and understand each module of the genetic circuit we designed two composite parts that allow us to verify the (1) the degradation rate of AHL by AiiA and (2) induction of the pLux promoter by LuxR in presence of AHL with and without co-expression of AiiA.(1) The here presented BioBrick consists of a constitutive promoter driving expression of AiiA and LuxR followed by a Lux promoter which drives expression of a fluorescent reporter (here: yellow fluorescent protein). For a schematic overview see the figure below
Notes
noneSource
parts registry and gBlocks ordered from idt| Sequence Annotation | Location | Component / Role(s) |
| BBa_I14032/p(Lac) IQ -35 -10 RBS/BBa_B0034 AiiA RBS LuxR BBa_B0010/term BBa_B0012/term BBa_R0062/pLux promoter | 1,37 3,8 26,31 44,50 59,812 820,832 838,1590 1627,1707 1715,1756 1764,1818 | promoter feature/promoter feature/misc sequence_feature feature/misc sequence_feature feature/rbs ribosome_entry_site feature/cds CDS ribosome_entry_site feature/rbs CDS feature/cds stem_loop feature/stem_loop stem_loop feature/stem_loop feature/promoter promoter |