MtrB

BBa_K1769005 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1769005
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ho-Tsang Tsai
Date created: 2015-09-14 11:00:00
Date modified: 2015-09-15 03:37:18

MtrB Outer Membrane Structural Protein



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K1769005_sequence (Version 1)

Description

MtrB, an outer membrane protein from Shewanella oneidensis MR-1, is required for proper incorporation of cytochromes into the outer membrane. This protein is important in stablizing OcmA and OcmB. two outer membrane protein that plays an essential role in netal reduction pathway in S.oneidensis and is also important in genetraing current in microbialfuel cell. We reintroduce this gene into a mtrB gene knockout mutant Shewanella JG700 to create a sensor with electric current as reporter. With a suitable inducible promoter, mtrB will be activated whenever the promoter is induced.

Notes

MtrB might slow down the growth of E.coli DH5alph and E.coli S-17.

Source

Shewanella oneidensis MR-1

Sequence Annotation Location Component / Role(s)
start codon
stop codon
1,3
2092,2094
feature/start start_codon
stop_codon feature/stop
igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1769005/1