BBa_K1773021

BBa_K1773021 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1773021

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sarunas Tumas

Date created: 2015-08-25 11:00:00

Date modified: 2015-08-26 03:55:15

Cas3 protein with pLuxcI right promoter and weak RBS.

Details

• Types: DnaRegion
• Roles: engineered_region; Generator
• Sequences: BBa_K1773021_sequence (Version 1)

Description

Gene encoding I-F type CRISPR_Cas system Cas3 protein, which is an helicase and ssDNA nuclease. Attached there is a pLux/cI right promoter and weak RBS site. In the I-F type CRISPR-Cas system Cascade complex distinguishes target DNA, and rectruits Cas3 protein, which acts as a nuclease and helicase, which hidrolises ssDNA in a 3'-5' direction.

Notes

no

Source

I-F type CRISPR-Cas system was cloned from Aggregatibacter actinomycetemcomitans D7-S strand.

Components

Access	Instance	Definition
public	BBa_K1773018	BBa_K1773018
public	BBa_K1773003	BBa_K1773003

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1773018	1,89	BBa_K1773018
BBa_K1773003	96,3381	BBa_K1773003

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1773021/1

Member of these Collections

• iGEM Parts Registry