Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Eduardo R. Serratos
Date created: 2015-09-17 11:00:00
Date modified: 2015-09-18 12:28:10
High Cys-Thr protein
| Types | DnaRegion |
| Roles | sequence_feature Other |
| Sequences | BBa_K1787000_sequence (Version 1) |
Description
This part contains a T7 promoter with a Ribosome Binding Site next to it; additionally, it encodes for a protein which has a 26% composition of Cysteine and 20% composition of Threonine. We have preliminary data that suggests that after isolation, run through an SDS-PAGE, and silver staining, this protein shows a different colouration than that of the normal silver stained protein gels.Notes
In order for our sequence to be synthesized, we had to intercalate non-polar amino acids between the cysteines and the threonines; these two being of a polar nature.Source
The DNA sequence encoding for the protein of interest was engineered in a way so as to have a higher Cysteine and Threonine concentration. It does not exist in Nature. It was introduced into BBa_K1321338 (containing a T7 promoter with a RBS).| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1321338 Scar (PstI/XbaI) High in Cys-Thr Protein | 1,43 45,50 51,629 | engineered_region feature/BioBrick feature/scar feature/protein CDS |