BBa_K1792005

BBa_K1792005 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1792005

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Brian Dempsey

Date created: 2015-09-16 11:00:00

Date modified: 2015-09-17 02:27:52

Nuclease - secreted with Cterm 6xHis tag

Details

• Types: DnaRegion
• Roles: Other; sequence_feature
• Sequences: BBa_K1792005_sequence (Version 1)

Description

This part exists as a basic part only. For a working part see the composite part: BBa_K1792004

Nuclease from Staphylococcus aureus. This nuclease is secreted due to a bacterial N-terminal export tag. The nuclease contains a C-terminal 6xHis tag for possible purification and concentration. This part is the same as BBa_K729004 with the addition of the his-tag. Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides (Enzyme that catalyzes the hydrolysis of both DNA and RNA at the 5' position of the phosphodiester bond.)

Notes

The first 64 amino acids have been removed (not necessary for function). It has a functional bacterial N-terminal secretion tag that is 19 amino acids in length.

Source

This is a genomic sequence of Nuclease from Staphylococcus aureus. Found in the UniprotKB database: P00644 (NUC_STAAU). The first 64 amino acids have been removed (not necessary for function). It has a functional bacterial N-terminal secretion tag that is 19 amino acids in length.

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1792005/1

Member of these Collections

• iGEM Parts Registry