BBa_K1795012

BBa_K1795012 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1795012
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andrew D Halleran, Joseph L Maniaci
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-17 09:46:36

I0500 gRNA under R0040



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K1795012_sequence (Version 1)

Description

This sgRNA sequence was designed to target the Promoter Bba_I0500 with the N20 sequence tttctccatacccgtttttt . If this gRNA is expressed in a cell with a form of the dCas9 protein, the protein will be targeted to the N20 sequence and repress gene expression. The part is under the control of the promoter Bba_R0040. Promoter Bba_R0040 was chosen because it does not contain a PAM and therefore cannot be inadvertently repressed by dCas9 due to unintentional homology in the N20 sequence. Expression of this part can be repressed if TetR is expressed in the cell without Tetracycline present. If TetR and Tetracycline (or its analog aTc) are both present, the part will be expressed.

Notes

We made sure to use a different promoter for this part than the one which the part targets.

Source

The basic template for the sgRNA sequence was taken from the supplementary information of Qi LS, Larson MH, Gilbert LA, et al. Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Cell. 2013;152(5):1173-1183. doi:10.1016/j.cell.2013.02.022.

igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1795012/1