Types | DnaRegion
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Roles | Measurement
engineered_region
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Sequences | BBa_K1796002_sequence (Version 1)
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Description
Pnif+amilCP reporter.
We ligated Pnif(BBa_K1796001) and amilCP reporter(BBa_K1357009) together to assay the validity and effeciency of Pnif. It worked. Picture is shown below.
http://2015.igem.org/wiki/images/thumb/3/34/C-confirmation_of_Pnif.jpeg/622px-C-confirmation_of_Pnif.jpeg
Notes
We conserved 100bp to insure it work. Actually, it can work with 50bp.
Source
Pnif is from the nitrogen fixation gene cluster of diazotroph Paenibacillus sp. WLY78,we standardized it.
amilCP reporter(BBa_K1357009) is from 2015 20D Kit5 .