| Types | DnaRegion
|
| Roles | Device
engineered_region
|
Description
This part contains a sgRNA which we designed to be expressed in procaryotic organism. There is a promoter in fron of it and a terminator behind.Compared to Part:BBa_K1796203, this part contains a certain crRNA targetted on pyruvate dehydrogenase (PDH) complex E1. So we can use this part to knockout E1 in procaryotic organism with CAS9 by the way of CRISPR. The constitutive promoter and the terminator are based on biobricks BBa_J23100 and BBa_B0012, respectively. To make these two parts more compatible with our sgRNA, we made some modifications on them. For the promoter, we changed -10 box into TATAAT, aiming at higher efficiency of transcription. What???s more, only the core domain of terminator B0012 which can form hairpin structure and oligo U were chosen. We have to make the part a short one to avoid undesired situations, for example, the sgRNA is too long to combine with Cas9 protein.
Notes
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Source
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