BBa_K1797010

BBa_K1797010 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1797010

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: iGEM15_Tsinghua

Date created: 2015-09-16 11:00:00

Date modified: 2015-09-18 07:03:06

Kana cassette flanked by loxP site

Details

• Types: DnaRegion
• Roles: Reporter; engineered_region
• Sequences: BBa_K1797010_sequence (Version 1)

Description

This part includes a translational kanamycin resistance gene(in prokaryotes) or a neomycin resistance gene(in eukaryotes) and flanking loxP sites. We can use DNA recombination technology to knock this part into E.coli genome and the antibiotic resistance gene helps us find the right colonies. Then if Cre is expressed in the bacteria, this kana cassette can be excised and a knock out experiment is completed.

Notes

No.

Source

No.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
LoxP site	2,35	sequence_feature, feature/misc
NeoR/KanR	403,1197	feature/protein, CDS
LoxP site	1210,1243	feature/misc, sequence_feature

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1797010/1

Member of these Collections

• iGEM Parts Registry