Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: iGEM15_Tsinghua
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-18 07:05:48
dCas9-Long Linker (Gly-Gly-Ser-Gly)x3-Flp
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1797016_sequence (Version 1) |
Description
In this part, we fuse dCas9(BBa_K1797006) and catalytic domain of Flp(BBa_K1015001) with a long linker(BBa_K243006). Flp is a site-specific recombinase. Due to its specific recognition site, the use of recombinase is limited. To solve this problem, we fuse its catalytic domain with dCas9. Then where the Flp is going to function is decided by gRNA.Notes
No.Source
No.| Sequence Annotation | Location | Component / Role(s) |
| dCas9 BBa_K1797006 Flp long lingker (Gly-Gly-Ser-Gly)x3 | 1,4107 1,4107 4141,5391 4105,4140 | feature/protein CDS feature/BioBrick engineered_region CDS feature/protein CDS feature/protein |