BBa_K1799005

BBa_K1799005 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1799005
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Miranda Halle
Date created: 2015-08-06 11:00:00
Date modified: 2015-08-07 12:37:51

LsrD (wild type)



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1799005_sequence (Version 1)

Description

Gene for the D subunit of the LsrACDB transporter, present in E. coli on the LsrACDBFG operon. The D subunit is a permease protein in the transporter, which actively brings autoinducer 2 (AI-2) into the cell.

Notes

The portion of the LsrACDBFG operon that codes for the AI-2 transporter consists of four open reading frames, which code for A, C, D, and B, respectively. Three of these ORFs overlap with one another: C overlaps with A, and its RBS is contained within the A subunit ORF, and D overlaps with C (although to a lesser extent), and its RBS is contained within the C subunit ORF. From a modularization standpoint, this creates an inefficiency because the wild type ORFs of the A and C subunits both contain functional RBSs, and in the case of the A subunit ORF, an ATG 4bp downstream. To reduce this inefficiency, we have created versions of each subunit ORF that are optimized for modularization. Further explanation is available in the descriptions of those parts.

Source

This part was synthesized from E. coli genomic data retrieved via EcoGene: http://www.ecogene.org/gene/EG13808

igem#status
Planning
 
rdf-schema#seeAlso
A8A068
 
rdf-schema#seeAlso
B1IRU5
 
rdf-schema#seeAlso
B1XEA3
 
rdf-schema#seeAlso
P0AFS2
 
rdf-schema#seeAlso
Q0T4L7
 
rdf-schema#seeAlso
P0AFS1
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1799005/1