BBa_K1799007

BBa_K1799007 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1799007

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Michael Flanagan

Date created: 2015-08-06 11:00:00

Date modified: 2015-09-18 05:03:23

LsrA (modularization optimized)

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1799007_sequence (Version 1)

Description

Gene for the A subunit of the LsrACDB transporter, present in E. coli on the LsrACDBFG operon. The A subunit is the ATP-binding domain of the transporter, which actively brings autoinducer 2 (AI-2) into the cell.

The portion of the LsrACDBFG operon that codes for the AI-2 transporter consists of four open reading frames, which code for A, C, D, and B, respectively. Three of these ORFs overlap with one another: C overlaps with A, and its RBS is contained within the A subunit ORF, and D overlaps with C (although to a lesser extent), and its RBS is contained within the C subunit ORF. From a modularization standpoint, this creates an inefficiency because the wild type ORFs of the A and C subunits both contain functional RBSs, and in the case of the A subunit ORF, an ATG 4bp downstream. To reduce this inefficiency, we have created versions of each subunit ORF that are optimized for modularization.

Notes

This version of the open reading frame has been optimized for modularization via silent mutations of the LsrC RBS and ATG, as well as any segments of code sharing more than 83% homology with the Shine-Dalgarno sequence. It is otherwise identical to the wild type LsrA gene (http://parts.igem.org/Part:BBa_K1799003).

Source

This part was synthesized, and is based on E. coli genomic data retrieved via EcoGene: http://www.ecogene.org/gene/EG13806

Other Properties

• igem#experience: None
• igem#status: Planning
• rdf-schema#seeAlso: P77257
• rdf-schema#seeAlso: B1XEA1
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1799007/1

Member of these Collections

• iGEM Parts Registry