Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Michael Flanagan
Date created: 2015-08-06 11:00:00
Date modified: 2015-09-18 05:03:23
LsrA (modularization optimized)
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1799007_sequence (Version 1) |
Description
Gene for the A subunit of the LsrACDB transporter, present in E. coli on the LsrACDBFG operon. The A subunit is the ATP-binding domain of the transporter, which actively brings autoinducer 2 (AI-2) into the cell.The portion of the LsrACDBFG operon that codes for the AI-2 transporter consists of four open reading frames, which code for A, C, D, and B, respectively. Three of these ORFs overlap with one another: C overlaps with A, and its RBS is contained within the A subunit ORF, and D overlaps with C (although to a lesser extent), and its RBS is contained within the C subunit ORF. From a modularization standpoint, this creates an inefficiency because the wild type ORFs of the A and C subunits both contain functional RBSs, and in the case of the A subunit ORF, an ATG 4bp downstream. To reduce this inefficiency, we have created versions of each subunit ORF that are optimized for modularization.