BBa_K180000

BBa_K180000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K180000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Richard Wilson, Stephanie Chambers
Date created: 2009-10-11 11:00:00
Date modified: 2015-05-08 01:11:06

Hybrid promoter (trp & lac regulated -- tac pR)



    • Details

    Types
    DnaRegion

    Roles
    promoter

    Regulatory

    Sequences BBa_K180000_sequence (Version 1)

    Description

    The Ptac promoter is a functional hybrid promoter, derived from the trp and lac promoters, that are regulated by trp and lac [1]. The Ptac sequence has been extracted by PCR from the commercially available plasmid pMAL-pIII from New England Biolabs (N8101S), with the addition of the biobrick prefix and suffix sequences.

    [1] Proc. Natl. Acad. Sci. USA, Vol. 80, pp. 21-25

    Notes

    Sequence includes lacIq and Shine-Dalgarno sequence from pMAL-pIII prior to the Ptac promoter to ensure correct functionality of the promoter.

    Source

    pMAL-pIII (New England Biolabs N8101S)

    Sequence Annotation Location Component / Role(s)
    lacIq
    Ptac
    		1,1082
    1325,1352
    		CDS feature/protein
    feature/promoter promoter

    igem#experience
    None
     
    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K180000/1
     

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