Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Arnas Petrauskas
Date created: 2015-09-15 11:00:00
Date modified: 2015-09-18 06:12:06
ALDH1 from Artemisia annua, 6 His tagged
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1808004_sequence (Version 1) |
Description
This is the coding region of the ALDH1 gene from Artemisia annua with added biobrick suffix, prefix and a 6 His tag in a pSB1C3 plasmid backbone. This part is an element of TCD iGEM 2015 set of parts for the synthesis of the anti-malarial drug precursor dihydroartemisinic acid in E. coli. The ALDH1 gene encodes the enzyme aldehyde dehydrogenase 1, which catalyses the conversion of (11R)-dihydroartemisinic aldehyde to dihydroartemisinic acid as well as the conversion of artemisinic aldehyde to artemisinic acid with NADP+ as the substrate. Artemisinic acid and dihydroartemisinic acid can be extracted from E. coli cells through lysis and chemically converted to the anti-malarial artemisinin. This part is codon optimised for BioBrick 3A assembly and expression in E. coli cells as well as protein purification.Notes
This part is BioBrick 3A assembly, E. coli codon and synthesis optimised. This part is 6 His tagged to allow protein purification.Source
Artemisia annua| Sequence Annotation | Location | Component / Role(s) |
| ALDH1 6 His tagged 6 His | 1,1518 1497,1515 | feature/cds CDS feature/tag tag |