Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wenbo Xu, Hao Wu
Date created: 2015-09-04 11:00:00
Date modified: 2015-09-17 07:16:24
pBAD + RNA Thermometer A1
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1824003_sequence (Version 1) |
Description
This is a composite regulatory part that consisting of pBAD promoter and RNA Thermometer A1 BBa_K1824556 for temperature induced gene expression at 42 degrees. The possible secondary structure of A1 was simulated by RNAstructure (Fig.1). This combination is compatible with the assembly method that XJTLU-CHINA promoted.Notes
The transcription of promoters always came with redundancy, which means the last few nucleic acids of the promoter can also be transcribed. It is important to avoid truncation or alteration of the RNA sequenceSource
pBAD was from the Registry, and A1 was from BIT-CHINA.| Access | Instance | Definition |
| public public | BBa_I13453 BBa_K1824557 | BBa_I13453 BBa_K1824557 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I13453 BBa_K1824557 | 1,130 131,180 | BBa_I13453 BBa_K1824557 |