BBa_K1824556

BBa_K1824556 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1824556
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wenbo Xu
Date created: 2015-09-03 11:00:00
Date modified: 2015-09-05 05:40:08

RNA Thermometer A1 (Specially designed for J23119)



Types
DnaRegion

Roles
mature_transcript_region

RNA

Sequences BBa_K1824556_sequence (Version 1)

Description

A1 RNA thermometer was derived from the ROSE family (ROSE stands for Repression Of heat Shock gene Expression), first discovered in the 5′ untranslated region of rhizobial heat shock genes (Nocker, 2001). The possible secondary structure of A1 was simulated by RNAstructure (Fig.1). For testing results of A1 RNAT, See below.

Notes

To maintain the secondary structure of RNA thermometers, researchers need to be careful with of the use of any parts that couple with it. For instance, in some cases, part of operator region can be transcribed into RNAs and form tight secondary structure and thus interfere RNA thermometers.

Source

the DNA sequence of A1 was from BIT-CHINA.

igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1824556/1