| Types | DnaRegion
|
| Roles | RNA
mature_transcript_region
|
| Sequences | BBa_K1824564_sequence (Version 1)
|
Description
This is a specially designed U6 RNA thermometer that with a unique spacer at the front, which would make it compatible with promoter T7.It is important to noted that this part is not compatible with RFC[10]. XJTLU-CHINA used Gibson method to assembly this part to others.
Notes
To maintain the secondary structure of RNA thermometers, researchers need to be careful with of the use of any parts that couple with it. For instance, in some cases, part of operator region can be transcribed into RNAs and form tight secondary structure and thus interfere RNA thermometers.
Source
The DNA sequence of U6 was from registry.
