BBa_K1826000

BBa_K1826000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1826000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jayaprakash S

Date created: 2015-09-17 11:00:00

Date modified: 2015-09-18 04:58:56

Mutant pBAD/araC promoter containing SacI site

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K1826000_sequence (Version 1)

Description

This part is an improvement on the biobrick __. It carries a mutation in the form of the introduction of a SacI site in the base number 430. Substitution of C with G introduces a SacI site. We used this for assembling our parts into the pET 24a vector.

Notes

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Source

The part was derived from the sequence of BBa_I0500 and partially ordered from IDT.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
mutant araC with SacI site	1,879	feature/cds, CDS
pBAD	906,1170	promoter, feature/promoter

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1826000/1

Member of these Collections

• iGEM Parts Registry