| Types | DnaRegion
|
| Roles | engineered_region
Composite
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| Sequences | BBa_K1827000_sequence (Version 1)
|
Description
This part consists of the three following genes extracted from E.coli: lpp signal sequence, lpp gene, and ompA gene. The lpp signal sequence allows the lpp and ompA sequence to be expressed successfully. The lpp(lipoprotein) gene that is used in this part consists of the first 9 amino acids of lpp gene, which can still be expressed as proper-functioning lipoprotein. The ompA gene starts from the 46th amino acid to 159th amino acid of the original ompA gene. The whole part is used for creating a specific protein complex called lpp-ompA complex, present in the cellular membrane of E.coli. This complex can attach almost every molecule to the membrane, thereby serving as an effective biotechnological and industrial tool with great potential. The users of this part can insert adequate genes (whether those are from E.coli or from other species) next to this sequence to give E.coli certain function that does not exist in normal E.coli. Thus, they can use this part as a basis to design their own E.coli, suitable for their purpose. As it allows signaling molecules and enzymes to be attached with ease, it has a wide variety of applications, from detection of specific heavy metal to breakdown of macro-molecules. This part, although already used by some people, is meaningful and economical in that it contains only the necessary parts in expressing the protein complex that can be used in various ways by other people. Also, it allows diverse extracellular processes to be carried out without interrupting the inner mechanisms of E.coli, since the complex presents the molecule outside the cell.
Notes
The additional design considerations were not necessary.
Source
The three components of this part come from separate parts of the E.coli genome. The genes were extracted from grown E.coli through conventional laboratory methods such as DNA mini-prep. Those three genes were then ligated into a single part that has certain function as a whole.
