| Types | DnaRegion
|
| Roles | polypeptide_domain
Protein_Domain
|
| Sequences | BBa_K1829000_sequence (Version 1)
|
Description
The Bar1 gene codes for the production of Bar1, a protease that degrades the yeast mating pheromone alpha factor. It has homology to aspartic proteases and has endopeptidase action that cleaves alpha factor between 6-Leu and 7-Lys. Bar1 is secreted into the periplasmic space between the cell membrane and cell wall of MATa S. cerevisiae. It is heavily glycolysated and maintained in the transmembrane space in yeast cells, where it creates a pheromone sinkhole that desensitizes yeast cells to alpha factor signal and sharpens concentration gradients of alpha factor.
Notes
We changed nucleotide 105 from cytosine to thymine to eliminate an EcoR1 cutsite. This does not change the amino acid (phenylalanine) therefore it is a silent mutation. We also changed nucleotide 1575 from thymine to cytosine. This does not change the amino acid (Asparagine) therefore it is a silent mutation.
Source
Bar1 is found in the genomic sequence of MATa S. cerevisiae.
