BBa_K1833010

BBa_K1833010 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1833010
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Konstantin Borisov
Date created: 2015-09-17 11:00:00
Date modified: 2015-09-18 07:44:36

pT7-MMP2 sensitive repressor



Types
DnaRegion

Roles
engineered_region

Generator

Sequences BBa_K1833010_sequence (Version 1)

Description

This part produces a synthetic repressor in response to T7 RNA Polymerase. This synthetic repressor consists of a fusion of two DNA binding domains connected with a linker that is sensitive to matrix metalloprotease 2, and it represses the promoter described by parts BBa_K1833001 and BBa_K1833003. For more information on the use of this part, visit the 2015 Pitt iGEM page.

Notes

made it

Source

found it

Sequence Annotation Location Component / Role(s)
pT7
rbs
start
434 cI N-terminus
434 cI N-terminus with P22 specificity
stop
MMP2 sensitive linker
T7 terminator
1,20
24,29
33,35
33,338
435,713
714,719
339,434
720,760
feature/promoter promoter
ribosome_entry_site feature/rbs
feature/start start_codon
CDS feature/protein
CDS feature/protein
stop_codon feature/stop
CDS feature/protein
feature/stem_loop stem_loop
igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1833010/1