Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Anthony Ciesla
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-17 03:04:36
Empty gRNA Scaffold
| Types | DnaRegion |
| Roles | Other sequence_feature |
| Sequences | BBa_K1835000_sequence (Version 1) |
Description
Using the restriction enzyme BsaI, an opening will be made to insert annealed oligonucleotides that can code your gRNA.Notes
The BsaI sites were devised with enough space to avoid interference and designed to cut themselves out from the produced backbone.Source
The part has been in use in our lab originally from the Luciano Marrafini Lab originating from S. Peumoniae.| Sequence Annotation | Location | Component / Role(s) |
| Repeat Repeat Loop gRNA Oligos Binding Site gRNA Oligos Binding Site | 20,55 86,122 53,56 82,85 | feature/stem_loop stem_loop stem_loop feature/stem_loop start_codon feature/start feature/stop stop_codon |