Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Connor McFadden
Date created: 2015-09-17 11:00:00
Date modified: 2016-01-21 02:20:44
MLC Glucose Sensitive Yellow Chromoprotein Construct
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1838005_sequence (Version 1) |
Description
This composite part features our MLC2 construct combined via 3A assembly with a yellow chromoprotein+RBS part (BBa_K1033931). During transcription, the MLC2 binding site is bound by the protein MLC, inhibiting production of yellow chromoprotein. As glucose concentration increases in the cell, more MLC is uptaken and bound to the membrane by a mechanism involving the transmembrane glucose permease called IICBGlu. This means that higher glucose concentrations subsequently result in higher expression levels of the yellow chromoprotein and thus makes it a glucose-inducible chromoprotein producing part. Specifically, this part tests the hypothesis for how MLC affects the inducibility of a strong promoter when placed downstream of the base promoter sequence.Notes
One issue to consider was where to place the MLC binding site and what strength promoter to use as a base. This was solved by creating multiple variants of the promorer (MLC1-4). This part may be improved by considering how the RBS is affected by the position of the MLC binding site.Source
1. Plumbridge, Jacqueline. ???DNA Binding Sites for the MLC and NagC Proteins: Regulation of nagE, Encoding the N-Acetylglucosamine-Specific Transporter inEscherichia Coli.??? Nucleic Acids Research 29.2 (2001): 506???514. Print.2. http://parts.igem.org/Part:BBa_J23110
3. http://parts.igem.org/Part:BBa_K1033931
| Access | Instance | Definition |
| public public | BBa_K1838001 BBa_K1033931 | BBa_K1838001 BBa_K1033931 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1838001 BBa_K1033931 | 1,58 67,783 | BBa_K1838001 BBa_K1033931 |