Types | DnaRegion
|
Roles | Coding
CDS
|
Sequences | BBa_K184000_sequence (Version 1)
|
Description
Silicanization protein Silintaphin-1.
Notes
Synthetic gene assembly of Silintaphin-1 was created by splitting the Silintaphin-1 gene into (2) separate parts because of high GC content and repeat sequences introduced by the (GS4)6 linker.
Source
Silintaphin-1 was created by synthetic gene synthesis with silent mutations to delete biobrick cloning sites in the endogeneous gene sequence. The gene was also codon optimised for human and e.coli expression. The Silintaphin-1 gene was derived from the silicanaceous sponge, Suberites domuncula.