BBa_K1840001

BBa_K1840001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1840001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Julia Anna Adrian

Date created: 2015-09-17 11:00:00

Date modified: 2015-09-18 01:48:26

Kgu promotor from Pseudomonas putida

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K1840001_sequence (Version 1)

Description

This basic part is the promotor in front of four so called kgu genes. They all are associated with 2-ketogluconate (2-ketogluconate epimerase, kinase, transporter and reductase) in Pseudomonas putida. All of them are concerned with the glucose metabolism in P. putida. The kgu promotor is negatively regulated by the repressor called PtxS. The glucose derivative 2-Ketogluconate can bind to it and this leads to subsequent release of PtxS from the promotor.
For our project (NTNU Trondheim 2015) we integrated the promotor in front of the mCherry gene. Hence we built a glucose detection device, that expresses mCherry according to the glucose (and therefore 2-Ketogluconate) level in the environment.
Note: The promotor gad (BBa_K1840003) is repressed by the same repressor PtxS. See biobrick parts BBa_K1840000 and BBa_K1840002 for other promotors on operons regulated by glucose derivatives.

Notes

The considerations we made regarded the starting and endpoint of the promotor. We only know that it has to be in front of the kgu gene. We assumed it starts directly after the previous gene. This gene codes for PtxS, the repressor.

Source

This part was designed after information from the paper by DADDAOUA, A., KRELL, T., ALFONSO, C., MOREL, B. & RAMOS, J.-L. 2010. Compartmentalized Glucose Metabolism in Pseudomonas putida Is Controlled by the PtxS Repressor. Journal of Bacteriology, 192, 4357-4366.

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1840001/1

Member of these Collections

• iGEM Parts Registry