Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Julia Anna Adrian
Date created: 2015-09-17 11:00:00
Date modified: 2015-09-18 11:18:51
Device: TT-edd-mCherry
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1840005_sequence (Version 1) |
Description
This device acts as a detector for glucose (and certain derivatives of it). The double transcription terminator ensures, that only the gene downstream of edd is influenced by the promotor edd. The edd promotor (BBa_K1840000) is contained in the genome of Pseudomonas putida and is situated in front of the enzyme edd (6-phosphogluconate dehydratase). The repressor HexR can bin the edd promotor sequence and thus prevent the transcription of the edd gene. In case the glucose derivative 2-Keto-3-deoxy-6-phosphogluconate is present, it binds the promotor and leads to a subsequent conformational change. Hence, the repressor can no longer bind and the gene downstream can be transcribed. In our device, the downstream gene is mCherry, codon optimized for Pseudomonas (BBa_K1840004). Therefore, in the presence of glucose, mCherry is expressed. In various experiments, we could see that this device is in deed working.Notes
As a promotor sequence, we assumed all DNA in front of the edd gene up to the next gene, coding for gap-1. Since, it is encoded on the opposite strand, the is the possibility, that edd functions as a promotor for this gene as well. Therefore the double terminator was needed.Source
The double terminator was already registered in the iGEM catalogue. The edd comes from the genome of Pseudomonas putida. The mCherry is codon optimized for expression in Pseudomonas.| Access | Instance | Definition |
| public public public | BBa_B0014 BBa_K1840000 BBa_K1840004 | BBa_B0014 BBa_K1840000 BBa_K1840004 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_B0014 BBa_K1840000 BBa_K1840004 | 1,95 104,367 374,1087 | BBa_B0014 BBa_K1840000 BBa_K1840004 |