Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Luba Prout, Vitor Pinheiro
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-20 01:34:21
cI-Cro bacteriophage lambda lytic cycle regulatory circuit
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1846005_sequence (Version 1) |
Description
The cI-Cro construct contains a circuit for the production of the cI repressor protein (also known as the Lambda Repressor), which is responsible for keeping bacteriophage lambda in the lysogenic cycle through the cooperative binding of two repressor dimers to the DNA, repressing the Cro gene. The circuit uses a T7 TE promoter. This BioBrick also contains a circuit for the production of Cro governed by a T7 promoter, which turns off gene transcription during the lytic cycle.Notes
...Source
...| Sequence Annotation | Location | Component / Role(s) |
| BBa_B0012 T7 TE polya stop P(Cat) -35 -10 BBa_I14033 conserved BBa_B0031 BBa_K1846006 BBa_K648028 conserved BBa_B0034 BBa_I719005 BBa_B0010 stem_loop | 1,41 8,27 28,41 34,34 50,87 53,58 76,81 50,87 102,105 96,109 116,829 836,1036 1049,1052 1045,1056 1065,1087 1096,1175 1107,1150 | feature/BioBrick engineered_region feature/stem_loop stem_loop polyA_site feature/polya stop_codon feature/stop feature/operator operator feature/promoter promoter promoter feature/promoter engineered_region feature/BioBrick feature/conserved conserved_region feature/BioBrick engineered_region feature/BioBrick engineered_region feature/BioBrick engineered_region conserved_region feature/conserved feature/BioBrick engineered_region engineered_region feature/BioBrick engineered_region feature/BioBrick feature/stem_loop stem_loop |