Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Iain Galpin
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-17 03:12:50
INP-Zif268
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1855004_sequence (Version 1) |
Description
A fusion protein of a transmembrane domain (INP) and a zinc-finger domain (Zif268 1AAY fragment),under pL_lac promotion and containing a C-terminal FLAG tag. It is used to exhibit a zinc-finger dsDNA binding domain complimentary to CCGCCCNCGC on the cell surface of E.coli.Notes
We had to optimise the codon for E.coli K12, and then alter the codon slightly to remove unwanted cut sites. The INP domain is preceeded by an NdeI cut site, and followed by an AgeI cut site. The Zif268 fragment domain is preceeded by an NheI cut site and followed by a ClaI cut site.Source
Lpp-OmpA is originally from Pseudomonas syringae; Zif268 is from mice.| Sequence Annotation | Location | Component / Role(s) |
| pL_lac B0034 Strong RBS INP Linker Zif268 Linker 3x FLAG BBa_K864600 T0 Terminator | 1,56 60,80 84,1007 1011,1046 1053,1319 1326,1349 1353,1424 1431,1482 | promoter feature/promoter feature/rbs ribosome_entry_site CDS feature/protein sequence_feature feature/misc feature/protein CDS feature/misc sequence_feature feature/tag tag feature/stop stop_codon |