BBa_K1857001

BBa_K1857001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1857001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Joshua Timmons

Date created: 2015-09-15 11:00:00

Date modified: 2015-09-18 02:47:01

BBa_K1857001

Details

• Types: DnaRegion
• Roles: Other; sequence_feature
• Sequences: BBa_K1857001_sequence (Version 1)

Description

This resistance cassette confers resistance to Hygromycin B. Its resistance gene, Aph7", is under an HSP70A-RBCS2 fusion promoter, with an RBCS2 intron for greater expression, and before an RBCS2 3' UTR.

Berthold P, Schmitt R, Mages W (2002) An engineered Streptomyces hygroscopicus aph 7″ gene mediates dominant resistance against hygromycin B in Chlamydomonas reinhardtii. Protist 153:401-12

Notes

N/A

Source

This part was adapted from pOpt_mVenus_Hyg from ChalmyCollection.org.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
pHSP70A	1,268	feature/promoter, promoter
pRBCS2	275,496	feature/promoter, promoter
Aph7"	655,1653	sequence_feature, feature/misc
tRBCS2	1663,1896	feature/stop, stop_codon

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/myers
• synbiohub#ownedBy: user/james
• synbiohub#topLevel: BBa_K1857001/1

Member of these Collections

• iGEM Parts Registry