Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Stefan Oehler
Date created: 2015-09-15 11:00:00
Date modified: 2015-09-18 04:51:49
Promoter+RibosomeBindingSite+NrfA (in pSB1A3)
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1866000_sequence (Version 1) |
Description
The assimilatory Nitrite Reductase gene under the control of a strong Promoter Anderson J23104 with a Ribosome Binding Site was cloned onto the pSB1A3 plasmid backbone. This gene catalyses the conversion of Nitrite (NO2 -) to Ammonium (NH4 +).The entire cassette can be excised from the backbone and used as per requirement.
Notes
None. The sequence is compatible with the 3A assembly recommended by iGEM.Source
The Promoter+RBS part was obtained from the Biobrick BBa_K880005.The gene was isolated from Pseudomonas aeruginosa genome. (Refer Biobrick BBa_K1153001)
| Access | Instance | Definition |
| public public | BBa_K880005 BBa_K1153001 | BBa_K880005 BBa_K1153001 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K880005 BBa_K1153001 | 1,55 62,1498 | BBa_K880005 BBa_K1153001 |