Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shien Yang Lee
Date created: 2015-09-15 11:00:00
Date modified: 2015-09-16 12:36:09
pro_LldD_term
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1867001_sequence (Version 1) |
Description
E. coli L-lactate dehydrogenase with constitutive promoter and terminator. Used for interconversion between pyruvate and L-lactate, which is a chemical attractant for mosquitoes. Part contains entire transcription cassette and only needs to be transformed directly into E. coli to function.Notes
The gene was placed under control of a strong constitutive promoter and terminator to encourage high levels of expression to maximise amount of L-lactate secreted.Source
Coding sequence from Ecocyc database, accessed at: http://biocyc.org/ECOLI/sequence?object=EG11963The coding sequence was synthesized as a Biobrick compatible part, which was then assembled to attach a promoter+rbs upstream and a terminator downstream. Promoter+rbs and terminator were registry parts BBa_K608002 and BBa_B0015 respectively.
| Access | Instance | Definition |
| public public | BBa_K608002 BBa_B0015 | BBa_K608002 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K608002 BBa_B0015 | 1,55 64,192 | BBa_K608002 BBa_B0015 |