Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shien Yang Lee
Date created: 2015-09-15 11:00:00
Date modified: 2015-09-16 01:03:24
pro_TnaA_TnaB_term
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1867024_sequence (Version 1) |
Description
TryptophanaseA and TryptophanaseB coding sequences under control of single constitutive promoter and RBS. Followed by double terminator. Used to increase production of indole in E. coli by increasing rate of tryptophan uptake and degradation via pyruvate pathway.Notes
Had to sequence optimize to remove Biobrick restriction sites from sequence.Source
Coding sequences from Ecocyc database, accessed at:http://biocyc.org/ECOLI/sequence?object=EG11005 and
http://biocyc.org/ECOLI/sequence?object=EG11006
| Access | Instance | Definition |
| public public public public | BBa_K608002 BBa_K1867021 BBa_K1867022 BBa_B0015 | BBa_K608002 BBa_K1867021 BBa_K1867022 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K608002 BBa_K1867021 BBa_K1867022 BBa_B0015 | 1,55 62,1477 1484,2731 2740,2868 | BBa_K608002 BBa_K1867021 BBa_K1867022 BBa_B0015 |