BBa_K1869002

BBa_K1869002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1869002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Conary Meyer

Date created: 2015-09-17 11:00:00

Date modified: 2015-09-18 02:30:05

Inducible CFA Synthase

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K1869002_sequence (Version 1)

Description

This is a composite part of BBa_R0010, BBa_B0034, and BBa_K1869000 designed to allow for IPTG inducible transcription of Cyclopropane Fatty Acid (CFA) Synthase. This composite part is intended to demonstrate the effect of the CFA synthase in vivo. It can be used to increase the acid resistance of the transformed organism by changing the phospholipid profile to include CFAs which decreases the membrane's permeability to weak acids.

Notes

Part BBa_K1869000 was inserted down stream of BBa_J04500 using Gibson cloning.

Source

This source was generated by joining parts BBa_J04500 and BBa_K1869000.

Components

Access	Instance	Definition
public	BBa_J04500	BBa_J04500
public	BBa_K1869000	BBa_K1869000

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J04500	1,220	BBa_J04500
BBa_K1869000	227,1378	BBa_K1869000

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1869002/1

Member of these Collections

• iGEM Parts Registry