Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Team Biobytes
Date created: 2009-10-16 11:00:00
Date modified: 2015-05-08 01:11:07
Sigma 70 75% promoter in pBA, Biobytes plasmid
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K187013_sequence (Version 1) |
Description
The 75% promoter produces ~1% of the expression level from a consensus promoter. The pBA plasmid (part BBa_K187000) adapts the promoter for assembly with other parts using the BioBytes assembly system. See the BioBytes BBF RFC for details on this method.Notes
The 75% promoter produces ~75% of the expression level from a consensus promoter. The pBA plasmid adapts the promoter for assembly with other parts using the BioBytes assembly system. See the BioBytes RFC for details on this method.The Biobytes assembly method requires that the DNA fragments to be assembled have long sticky ends of a particular sequence. DNA fragments with these sticky ends can be prepared using genes in pBA or pAB as a template. If the DNA cloned into pAB begins with a start codon, the structure of pAB and pBA will result in a ribosome binding site being included 7bp upstream of the start codon. It is not necessary to include a promoter or ribosome binding site with the gene when cloning it into pAB or pBA. Instead, a promoter with long sticky ends can be prepared from separate pAB and pBA plasmids just containing promoters. The desired promoter can then be assembled upstream of a gene using the BioBytes method. This ability to easily 'mix and match' promoters and genes allows gene expression to be standardized and optimized.
The addition of one DNA segment with the BioBytes method takes only 20 minutes.