| Types | DnaRegion
|
| Roles | Coding
CDS
|
| Sequences | BBa_K187016_sequence (Version 1)
|
Description
Kanamycin resistance can be used as a selectable marker on kanamycin containing plates. This kanamycin resistance gene is present in plasmid pAB (part BBa_K187000), which is one of two universal plasmids for the BioBytes assembly method. For details of the BioBytes method, see its BBF RFC.
Notes
This plasmid includes only the open reading frame (start codon to stop codon) of the kan resistance gene. A promoter and terminator are not included. pAB and pBA do include an RBS consensus sequence positioned 8 bp upstream of the ATG. We recommend that to express kan resistance, you use the Biobytes assembly method together with the promoter and terminator parts we have submited in pAB and pBA to assemble promoters and terminators onto the kan resistance gene. As there is a range of promoters to chose from, this allows rapid manipulation of gene expression level. Using the Biobytes method, several DNA segments can be combined in just 20min per segment.
Source
The kanamycin resistance gene was cloned out of the plasmid pKD4. Biobytes plasmid pAB is entered as BBa_K187000.
