BBa_K187365

BBa_K187365 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K187365
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Team Biobytes
Date created: 2009-10-19 11:00:00
Date modified: 2015-05-08 01:11:12

pAB_F (A end) USER primer for BioBytes plasmid pAB



Types
DnaRegion

Roles
Primer

primer

Sequences BBa_K187365_sequence (Version 1)

Description

This is the forward primer of a pair of universal primers for amplifying any gene out of pAB (BBa_187000). These primers contain uracil deoxyribonucleotides, allowing for digestion with the USER system which can be purchased from New England Biolabs (NEB). This produces A and B overhangs and finishes the production of the Bytes. With the gene contained within the pAB and pBA plasmids, Bytes can be easily stored and amplified using the universal primers at any time.

See the BioBytes RFC for details of the BioBytes assembly method.

Notes

See the BioBytes RFC for details.

Source

Oligonucleotide sequencing.

Sequence Annotation Location Component / Role(s)
This should be a uracil not thymine
This should be a uracil not thymine
7,7
12,12
sequence_feature feature/misc
sequence_feature feature/misc
igem#experience
Works
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K187365/1