Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Team Biobytes
Date created: 2009-10-19 11:00:00
Date modified: 2015-05-08 01:11:12
pAB_F (A end) USER primer for BioBytes plasmid pAB
| Types | DnaRegion |
| Roles | Primer primer |
| Sequences | BBa_K187365_sequence (Version 1) |
Description
This is the forward primer of a pair of universal primers for amplifying any gene out of pAB (BBa_187000). These primers contain uracil deoxyribonucleotides, allowing for digestion with the USER system which can be purchased from New England Biolabs (NEB). This produces A and B overhangs and finishes the production of the Bytes. With the gene contained within the pAB and pBA plasmids, Bytes can be easily stored and amplified using the universal primers at any time.See the BioBytes RFC for details of the BioBytes assembly method.
Notes
See the BioBytes RFC for details.Source
Oligonucleotide sequencing.| Sequence Annotation | Location | Component / Role(s) |
| This should be a uracil not thymine This should be a uracil not thymine | 7,7 12,12 | sequence_feature feature/misc sequence_feature feature/misc |