BBa_K187367

BBa_K187367 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K187367

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Team BioBytes

Date created: 2009-10-19 11:00:00

Date modified: 2015-05-08 01:11:12

pBA_F (B end) USER primer for BioBytes plasmid pBA

Details

• Types: DnaRegion
• Roles: Primer; primer
• Sequences: BBa_K187367_sequence (Version 1)

Description

This is the forward primer of a pair of universal primers for amplifying any gene out of pBA (BBa_187001). These primers contain uracil deoxyribonucleotides, allowing for digestion with the USER system which can be purchased from New England Biolabs (NEB). This produces A and B overhangs and finishes the production of the Bytes. With the gene contained within the pAB and pBA plasmids, Bytes can be easily stored and amplified using the universal primers at any time.

See the BioBytes RFC for details of the BioBytes assembly method.

Notes

See the BioBytes RFC for details.

Source

Oligonucleotide sequencing.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
should be uracil not thymine	12,12	feature/misc, sequence_feature
should be uracil not thymine	6,6	sequence_feature, feature/misc

Other Properties

• igem#experience: Works
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K187367/1

Member of these Collections

• iGEM Parts Registry