BBa_K187420

BBa_K187420 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K187420

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Julia Pon

Date created: 2009-10-20 11:00:00

Date modified: 2015-05-08 01:11:13

trpD in pBA

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K187420_sequence (Version 1)

Description

trpD encodes anthranilate synthase component II, which catalyses the second step of tryptophan biosynthesis.

This gene is present in plasmid pBA (part BBa_K187001), which is one of two universal plasmids for the BioBytes assembly method. For details of the BioBytes method, see RFC 47.

Notes

Note that the gene in this plasmid starts at the start codon and ends at the stop codon. pBA does not include a promoter or terminator. pBA does include an RBS consensus sequence positioned 8 bp upstream of the ATG. We recommend that to express this gene, you use the Biobytes assembly method together with the promoter and terminator parts we have submited in pAB and pBA to assemble promoters and terminators onto the gene. As there is a range of promoters to chose from, this allows rapid manipulation of gene expression level. Using the Biobytes method, several DNA segments can be combined in just 20min per segment. See RFC 47.

Source

MG1655 E.coli genomic DNA

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K187420/1

Member of these Collections

• iGEM Parts Registry